马德里竞技官宣:DON(Deoxynivalenol) ELISA Kit

    • Mycotoxin Detection ELISA Kit-Elabscience
    • Mycotoxin Detection ELISA Kit-Elabscience
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    >
    • Mycotoxin Detection ELISA Kit-Elabscience
    • Mycotoxin Detection ELISA Kit-Elabscience
    • Mycotoxin Detection ELISA Kit-Elabscience
    • Mycotoxin Detection ELISA Kit-Elabscience

      马德里竞技对巴伦西亚 www.brxfuh.com.cn Catalog number:E-TO-E003

      Size:
      • 96T
      Qty:
      - +
      Price: $388

      Sensitivity: 3.00 ppb

      Sample type: Grain, Feed

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      Test principle

      This kit uses Indirect-Competitive-ELISA as the method. It can detect Deoxynivalenol (DON) in samples, such as rice, millet, flour and other crops/feed, etc. This kit is composed of ELISA Microplate, HRP conjugate, antibody, standard and other supplementary reagents. The micro-plate provided in this kit has been pre-coated with DON. During the reaction, DON in the samples or standard competes with DON on the solid phase supporter for sites of DON antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each micro plate well, and TMB substrate is for color development. There is a negative correlation between the OD value of samples and the concentration of DON. The concentration of DON in the samples can be calculated by comparing the OD of the samples to the standard curve.


      Technical indicator

      Sensitivity: 3 ppb (ng/mL)

      Reaction mode: 37, 30 min~30 min~15 min

      Detection limit: Grain and Feed---150 ppb

      Cross-reactivity: Deoxynivalenol (C15H20O6)---100%, 3-Acetyldeoxynivalenol (C17H22O6)---1%,

      Sample recovery rate: Grain and formula fodder---85%±15%


      Kits components

      Item

      Specifications

      ELISA Microplate

      96 wells

      Standard Liquid

      1 mL each

      (0 ppb, 3 ppb, 9 ppb, 27 ppb, 81 ppb, 243 ppb)

      HRP Conjugate

      11 mL

      Antibody Working Solution

      5.5 mL

      Substrate Reagent A

      6 mL

      Substrate Reagent B

      6 mL

      Stop Solution

      6 mL

      20×Concentrated Wash Buffer

      40 mL

      2×Reconstitution Buffer 

      50 mL

      Plate Sealer

      3 pieces

      Sealed Bag

      1 piece

      Manual

      1 copy


      Other supplies required

      Instrument: Microplate reader, Printer, Homogenizer, Oscillators, Centrifuge, Graduated pipette, Balance (sensibility 0.01 g).

      High-precision transferpettor: Single channel (20-200 μL, 100-1000 μL), Multichannel (300 μL).



      Storage and valid period

      Storage: Store at 2-8. Avoid freeze / thaw cycles.

      Valid Period: 1 year, expiration date is on the packing box.

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