欧冠皇家马德里vs马德里竞技:AFM1(Aflatoxin M1) ELISA Kit

    • Mycotoxin Detection ELISA Kit-Elabscience
    • Mycotoxin Detection ELISA Kit-Elabscience
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    >
    • Mycotoxin Detection ELISA Kit-Elabscience
    • Mycotoxin Detection ELISA Kit-Elabscience
    • Mycotoxin Detection ELISA Kit-Elabscience
    • Mycotoxin Detection ELISA Kit-Elabscience

      马德里竞技对巴伦西亚 www.brxfuh.com.cn Catalog number:E-TO-E012

      Size:
      • 96T
      Qty:
      - +
      Price: $360

      Sensitivity: 0.05 ppb

      Sample type: Urine

      Lead Time: 7~10 daysWelcome to order from local distributors.

      Add to cart Compare Bulk request Manual

      Test principle

      This kit uses Indirect-Competitive-ELISA as the method. It can detect Aflatoxin M1 (AFM1) in urine samples. This kit is composed of ELISA Microplate, HRP conjugate, antibody, standard and other supplementary reagents. The ELISA Microplate provided in this kit has been pre-coated with AFM1. During the reaction, AFM1 in the samples or standard competes with AFM1 on the solid phase supporter for sites of AFM1 antibody. Then Horseradish Peroxidase (HRP) conjugate is added to each micro plate well, and TMB substrate is for color development. There is a negative correlation between the OD value of samples and the concentration of AFM1. The concentration of AFM1 in the samples can be calculated by comparing the OD of the samples to the standard curve.


      Technical indicator

      Sensitivity: 0.05 ppb (ng/mL)

      Reaction mode: 25, 30 min15 min

      Detection limit: Urine---1.5 ppb

      Cross-reactivity: Aflatoxin M1 (AFM1) ---100%

      Sample recovery rate: Urine---85%±15%


      Kits components


       

      Item

      Specifications

      ELISA Microplate

      96 wells

      Standard Liquid

      1 mL each

      (0 ppb, 0.05 ppb, 0.15 ppb, 0.45 ppb, 1.35 ppb, 4.05ppb)

      HRP Conjugate

      5.5 mL

      Antibody Working Solution

      5.5 mL

      Substrate Reagent A

      6 mL

      Substrate Reagent B

      6 mL

      Stop Solution

      6 mL

      20×Concentrated Wash Buffer

      40 mL

      2×Reconstitution Buffer

      40 mL

      Plate Sealer

      3 pieces

      Sealed Bag

      1 piece

      Manual

      1 copy



      Other supplies required

      Instruments: Microplate reader, Printer, Homogenizer, Nitrogen Evaporators, Oscillators, Centrifuge, Graduated pipette, Balance (sensibility 0.01 g).

      High-precision transferpettor: Single channel (20-200 μL, 100-1000 μL), Multichannel (300 μL).

      Reagents: Acetonitrile, Deionized Water.


      Storage and valid period

      Storage: Store at 2-8℃. Avoid freeze / thaw cycles.

      Valid Period: 1 year, expiration date is on the packing box.

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